Forward primers

Tube no.

5' dye

Name

Sequence

µL*^§

1

FAM

IS6110 f1

IS6110 f2

ACGCTAATTACCCGGTTCAT

AATGCACTAGCCGAGACGAT

15

15

2

HEX

MAC f

CACACTATTGGGCCCTGAGACA

10

2

HEX

MFG f

CACACTATTGGGCTTTGAGA

10

2

NED

MKG f

CACACTRTTGGGTCCTGAGGCA^†

15

2

HEX

MUCOG f

CACACTGTTGGGTCCTGAGA

10

2

FAM

SCRO f

CACACTATTGGGCCCTGAGGC

10

1, 2

FAM

β-globin f

CATAAAAGTCAGGGCAGAGCCA

5

Reverse primers

1

-

IS6110 r1

IS6110 r2

CCCGGTTGATGTGGTCGTAG

CGCCGCAGTACTGGTAGAGG

15

15

2

-

MAC r

ATGCTCGCAACCACTATCCAAT

10

2

-

MCAG r

TGCTCGCAACCACTATCCA

10

2

-

ABSC r

TGCTCGCAACCACTATTCAG

10

1, 2

-

β-globin r

ACTTCATCCACGTTCACCTTGC

5