Size Product

Primer sequences (5ʼ-3ʼ)

Type

Primer Name

No.

First amplification consensus

897 bp

TAGCTCCTTTTAATGTATGG

VP7 Forward

9CON 1F

1

AACTTGCCACCATTTTTTCC

VP7 Reverse

VP7 R

2

Second amplification genotyping primers

158 bp

CAAGTACTCAAATCAATGATGG

G1

aBT1

1

244 bp

CAATGATATTAAACATTTTCTGTG

G2

Act2

2

464 bp

ACGAACTCAACACGAGARG

G3

G3-AUST

3

403 bp

CGTTTCTGGTGAGGAGTTG

G4

aDT4

4

651 bp

GTCACACCATTTGTAAATTCG

G8

aAT8

5

110 bp

GTTGATGTGACTAYAAATAC

G9

G9 or Mg9

6