|
| RecA Primer CCGTTTATGCGGAACACCTA TCGGGATTACCAAACATCAC Torriani et al. (2001) | Volume of components | Orf-hisG primer CTTCGTTATGATTTTACA CAATATCAACAATTCCAT Coroller et al. (1999) | Volume of components |
| 10× |
| 25 µl |
| 25 µl |
| dNTP mix 10 mM |
| 5 µl |
| 5 µl |
| Forward primer |
| 10 µl |
| 15 µl |
| Reverse primer |
| 10 µl |
| 15 µl |
| 25 mM Mgcl2 |
| 15 µl |
| 30 µl |
| Template DNA |
| 2 µl |
| 3 µl |
| Taq polymerase 5 U/µl |
| 1.5 µl |
| 2 µl |
| Water, nuclease free |
| 175 µl |
| 146 µl |
| Total volume |
| 250 µl |
| 250 µl |