| Genes | Condition/Duration | |
| mcr-1/mcr-2 | Carbapenemases | |
| Initiale denaturation | 95˚C/15min | 95˚C/15min |
| Denaturation | 93˚C/30sec | 94˚C/1min |
| Hybridation | 57˚C/1min | 58˚C/1min |
| Elongation | 72˚C/1min | 72˚C/1min |
| Final elongation | 72˚C/8min | 72˚C/5min |
| Number of de cycles | 30 | 32 |