EN 1040

EN 13727

Basic conditions

Representative use conditions

Mandatory strains

S. aureus

P. aeruginosa

S. aureus

P. aeruginosa

E. coli

E. hirae

Additional strains*

12 Gram+ 11 Gram−

11 Gram+ 10 Gram−

Composition of the tested antiseptic

Foaming solution including 0.2% benzalkonium chloride (CBK) and 0.5% chlorhexidine digluconate (CHX) (Dermobacter®)

Concentrations of the antiseptic solution tested

Pure (97%/80%) or diluted (10%)

Interfering substances


Sheep erythrocytes (3 mL∙L−1) + bovine serum albumin (3 g∙L−1)

Contact time/T˚

1 min at 20˚C

Bactericidal activity: log reduction required

≥ 5