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| EN 1040 | EN 13727 |
| Basic conditions | Representative use conditions | |
| Mandatory strains | S. aureus P. aeruginosa | S. aureus P. aeruginosa E. coli E. hirae |
| Additional strains* | 12 Gram+ 11 Gram− | 11 Gram+ 10 Gram− |
| Composition of the tested antiseptic | Foaming solution including 0.2% benzalkonium chloride (CBK) and 0.5% chlorhexidine digluconate (CHX) (Dermobacter®) | |
| Concentrations of the antiseptic solution tested | Pure (97%/80%) or diluted (10%) | |
| Interfering substances | - | Sheep erythrocytes (3 mL∙L−1) + bovine serum albumin (3 g∙L−1) |
| Contact time/T˚ | 1 min at 20˚C | |
| Bactericidal activity: log reduction required | ≥ 5 | |