S. No.

Conc. (µg/ml)

Flow Rate (ml/min)

Mobile Phase Ratio (Buffer:ACN)

Rt

Peak Area (Mean)

% RSD

1

4

1

61:39

3.18

370,778

1.36

2

4

1

59:41

3.17

366,232.34

1.01

3

4

0.9

60:40

3.18

3,666,373

0.72

4

4

1.1

60:40

3.19

356,159

0.95