Intraday Precision
S. No.	Conc. (µg/ml)	Morning			Afternoon			Evening
S. No.	Conc. (µg/ml)	Mean	Assay %	RSD %	Mean	Assay %	RSD %	Mean	Assay %	RSD %
1	8	712,954.34	100.125	0.69	717218	100.75	0.23	716,060.67	100.625	0.09
2	16	1,402,589.67	101.75	0.09	1,403,482.33	101.875	0.04	1,402,693	101.812	0.32
3	32	2,694,083.33	99.331	0.07	2,694,278.33	99.312	0.07	2,694,393	99.343	0.15
Interday Precision
S. No.	Conc. (µg/ml)	Day 1			Day 2			Day 3
S. No.	Conc. (µg/ml)	Mean	Assay %	RSD %	Mean	Assay %	RSD %	Mean	Assay %	RSD %
1	8	716,060.667	100.625	0.16	717,248.33	100.75	0.05	717,358.67	100.837	0.05
2	16	1,402,312.67	101.75	0.05	1,401,464.67	101.687	0.01	1,401,276.33	101.687	0.06
3	32	2,696,056	99.406	0.1	2,695,367	99.375	0.04	2,695,505.33	99.384	0.12