S. No.	Conc. (µg/ml)	Intraday Precision
		Morning			Afternoon			Evening
		Mean	Assay %	RSD %	Mean	Assay %	RSD %	Mean	Assay %	RSD %
1	25	0.058	101.71	1.72	0.0573	100.38	1.01	0.0567	99.23	1.02
2	50	0.109	99.42	0.92	0.110	100.38	0.91	0.1084	98.85	0.54
3	100	0.215	100.19	0.47	0.2143	99.85	0.27	0.2137	99.57	0.27
Interday Precision
S. No.	Conc. (µg/ml)	Day 1			Day 2			Day 3
S. No.	Conc. (µg/ml)	Mean	Assay %	RSD %	Mean	Assay %	RSD %	Mean	Assay %	RSD %
1	25	0.0570	99.80	1.75	0.0570	99.80	1.75	0.0564	98.67	1.02
2	50	0.1094	99.80	0.53	0.1090	99.42	0.92	0.1077	98.47	0.54
3	100	0.2154	100.38	0.27	0.2147	100.04	0.27	0.2144	99.90	0.27