Preparative Chromatographic Conditions

Column

X-Select HSS CN OBDTM (250 × 19 5 µ)

Mobile phase

Acetonitrile (B), 10 mM Ammonium bicarbonate (A)

Programme

Gradient

Flow rate

20 mL/min

Wavelength

280

Column temperature

Ambient

Injection volume

500 µL

Diluent

Mobilephase

Gradient programme

(Time/%B): 0/5, 4/5, 10/50, 10.1/98, 12/98, 12.1/5, 14/5

Source voltages

Capillary (kV)

3.5

Cone (V)

35

Source Temperatures

Disolvation Temperature (˚C)

350

Source Gas flow

Desolvation Gas flow (L/Hr)

650

Cone (L/Hr)

50

Analyser

LM Resolution

15

HM Resolution

15

Ion Energy

0.5

Mass range

150 - 850