Preparative Chromatographic Conditions | |
Column | X-Select HSS CN OBDTM (250 × 19 5 µ) |
Mobile phase | Acetonitrile (B), 10 mM Ammonium bicarbonate (A) |
Programme | Gradient |
Flow rate | 20 mL/min |
Wavelength | 280 |
Column temperature | Ambient |
Injection volume | 500 µL |
Diluent | Mobilephase |
Gradient programme | (Time/%B): 0/5, 4/5, 10/50, 10.1/98, 12/98, 12.1/5, 14/5 |
Source voltages | |
Capillary (kV) | 3.5 |
Cone (V) | 35 |
Source Temperatures | |
Disolvation Temperature (˚C) | 350 |
Source Gas flow | |
Desolvation Gas flow (L/Hr) | 650 |
Cone (L/Hr) | 50 |
Analyser | |
LM Resolution | 15 |
HM Resolution | 15 |
Ion Energy | 0.5 |
Mass range | 150 - 850 |