| Compound | Regression equation | R2 | SCa (ng∙g−1) | RCb (ng∙g−1) | MRc (%) | CV (%) |
| 17 β-estradiol | Y = 0.9774x + 1.5228 | 0.9977 | 2.0 | 1.9 | 95.0 | 7.08 |
| 10.0 | 9.7 | 97.0 | 1.72 | |||
| 20.0 | 20.6 | 103.0 | 4.29 | |||
| 100.0 | 106.1 | 106.1 | 6.65 | |||
| 200.0 | 193.7 | 96.85 | 3.81 | |||
| Estrone | Y = 0.9936x + 0.4184 | 0.9998 | 2.0 | 2.0 | 100.0 | 9.37 |
| 10.0 | 10.3 | 103.0 | 6.08 | |||
| 20.0 | 19.8 | 99.0 | 6.34 | |||
| 100.0 | 101.8 | 101.8 | 6.09 | |||
| 200.0 | 198.2 | 99.1 | 3.31 | |||
| 17 α-ethinylestradiol | Y = 0.9831x + 1.0892 | 0.9987 | 2.0 | 2.0 | 100.0 | 11.65 |
| 10.0 | 10.0 | 100.0 | 9.37 | |||
| 20.0 | 20.0 | 100.0 | 7.65 | |||
| 100.0 | 104.7 | 104.7 | 5.80 | |||
| 200.0 | 195.2 | 97.6 | 2.95 | |||
| Estriol | Y = 1.0056x − 0.3771 | 0.9998 | 2.0 | 2.1 | 105.0 | 9.58 |
| 10.0 | 9.6 | 96.0 | 4.26 | |||
| 20.0 | 20.5 | 102.5 | 8.39 | |||
| 100.0 | 98.2 | 98.2 | 2.95 | |||
| 200.0 | 201.7 | 100.9 | 2.10 |