| Compound | Spiked Conc. (ng∙g−1) | Recovered Conc. (ng∙g−1)a | Standard Deviation (ng∙g−1) | Coefficient of variation (%) | Mean Recovery (%)a |
| 17 β-estradiol | 6.0 | 6.4 | 0.36 | 5.59 | 107.33 |
| 60.0 | 62.2 | 3.18 | 5.10 | 103.70 | |
| 180.0 | 182.0 | 7.81 | 4.29 | 101.13 | |
| Estrone | 6.0 | 6.3 | 0.30 | 4.84 | 104.90 |
| 60.0 | 62.1 | 3.82 | 6.15 | 103.57 | |
| 180.0 | 177.8 | 9.61 | 5.40 | 98.80 | |
| 17 α-ethinylestradiol | 6.0 | 6.4 | 0.26 | 4.03 | 106.17 |
| 60.0 | 64.3 | 3.06 | 4.76 | 107.23 | |
| 180.0 | 193.5 | 9.34 | 4.81 | 107.49 | |
| Estriol | 6.0 | 6.1 | 0.21 | 3.45 | 100.97 |
| 60.0 | 61.2 | 1.85 | 3.03 | 101.93 | |
| 180.0 | 181.0 | 9.14 | 5.04 | 100.58 |