| Secondary metabolite | Name of test | Methodology | Result |
| Alkaloid | Dragendorff’s test | Spot a drop of extract on a small piece of pre-coated TLC plate. Spray the plate with Dragendorff’s reagent. | Orange spot |
| Glycoside | Kellar–Kiliani test | Add 2 mL filtrate with 1 mL of glacial acetic acid, 1 mL ferric chloride and 1 mL concentrated sulphuric acid | Green-blue coloration of solution |
| Tannin | Braemer’s test | 10% alcoholic ferric chloride will be added to 2 - 3 mL of methanolic extract (1:1) | Dark blue or greenish grey coloration of the solution |
| Terpenoid | Salkowski test −10% | 5 mL extract was added with 2 mL of chloroform and 3 mL of concentrated sulphuric acid | Reddish brown colour of interface |
| Steroid | Liebermann-Burchardt test | To 1 mL of methanolic extract, add 1 mL of chloroform, 2 - 3 mL of acetic anhydride, 1 to 2 drops of concentrated sulphuric acid. | Dark green coloration |
| Reducing sugar | Fehling test Frothing | Add 25 mL of diluted sulphuric acid to 5 mL of water extract in a test tube and boil for 15 mins. Then cool it and neutralize with 10% sodium hydroxide to pH 7 and 5 mL of Fehling solution. | Brick red precipitate |
| Flavonoid | Kellar-Shinoda test | To 2 - 3 mL of methanolic extract, add a piece of magnesium ribbon and 1 mL of concentrated hydrochloric acid | Pink red or red coloration of the solution |