| Specific groups | Primers name | Primer sequence |
| Bacteroidetes | Bfr-F | CTGAACCAGCCAAGTAGCG |
| Bfr-R | CCGCAAACTTTCACAACTGACTTA | |
| Firmicutes | Fir-F | GGAGYATGTGGTTTAATTCGAAGCA |
| Fir-R | AGCTGACGACAACCATGCAC | |
| Bifidobacteria | Bif-F | CCTACGGGAGGCAGCAG |
| Bif-R | ATTACCGCGGCTGCTGG | |
| Lactobacillus | Lac-F | AGCAGTAGGGAATCTTCCA |
| Lac-R | ATTTCACCGCTACACATG | |
| Clostridium leptum | Clept-F | GCACAAGCAGTGGAGT |
| Clept-R | CTTCCTCCGTTTTGTCAA |