| Study ID | Journal | Location | Duration of trial | Samples size | Interventions | Outcomes | conclusion |
| Haydardedeoglu 2017 | BJOC | Turkey | 2015.01-2015.06 | SLN-Group:40 DLN-Group:40 | SLN-Group: 17-G needle with single lumen (Cook) DLN-Group: 17-G needle with double lumen (Cook); follicular flushing with 10 mL culture medium for three times | No. of oocytes retrieved/No. of follicles aspirated, No. of MII oocyte, procedure time, FR, IR, PR, LBR | DLN is time consuming and has similar results comparing to SLN. |
| Horn 2017 | Hum Reprod | Germany | 2015.02-2016.03 | SLN-Group:39 DLN-Group:39 | SLN-Group: 17-G needle with single lumen (Gynetics®) DLN-Group: 17-G Steiner-Tan Needle® system with follicular flushing for three times | No. of oocytes retrieved/No. of follicles aspirated, procedure time, OPR/LBR, | The use of DLN is unlikely to benefit the prognosis of patients with poor ovarian response. |
| Levens 2009 | Fertil Steril | USA | 2007.04-2007.10 | SLN-Group:15 DLN-Group:15 | SLN-Group: 16-G Echotip® ovum aspiration needle with single lumen (Cook, K-J-ANC-16R-35) DLN-Group:16-G double lumen needles (Cook, K-OPSD-1635-B-S); follicular flushing with 2 mL sterile PBS once | No. of oocytes retrieved/No. of follicles aspirated, No. of MII oocyte, procedure time, FR, OPR | The results did not demonstrate improved oocyte recovery with DLN even among those most likely to benefit from its application. |
| Mok-Lin 2013 | Hum Reprod | USA | 12-month period | SLN-Group:25 DLN-Group:25 | SLN-Group: 16-G Echotip® ovum aspiration needle with single lumen (Cook) DLN-Group: 16-G Echotip® ovum aspiration needle with double lumen (Cook); follicular flushing with 5 mL culture medium for four times | No. of oocytes retrieved/No. of follicles aspirated, No. of MII oocyte, procedure time, FR, IR, PR, LBR | DLN in the poorest responders does not increase the number of oocytes retrieved and may result in lower implantation and clinical pregnancy rates. |