| MS/MS conditions | Mitragynine | 7-hydroxymitragynine |
| Polarity | Positive | Positive |
| Ionspray voltage | 4000 V | 4000 V |
| Temperature | 550˚C | 550˚C |
| Collision gas | Medium | Medium |
| Ion source gas 1 | 50.0 | 55.0 |
| Ion source gas 2 | 65.0 | 65.0 |