| Primer | Sequences | PCR products (bp) | Ref. |
| MSP |
|
|
|
| SOCS1 |
|
|
|
| Unmethylated | F; TGAAGATGGTTTTGGGATTTATGA | 184 | [35] |
|
| R; CACAACTCCTACAACAACCACACAC |
|
|
| Methylated | F; TGAAGATGGTTTCGGGATTTACGA | 183 |
|
|
| R; ACAACTCCTACAACGACCGCACG |
|
|
| SOCS3 |
|
|
|
| Unmethylated | F; TAGTGTGTAAGTTGTAGGAGAGTGG | 134 | [35] |
|
| R; CTAAACATAAAAAAATAACACTAATCCAAA |
|
|
| Methylated | F; GTAGTGCGTAAGTTGTAGGAGAG | 139 |
|
|
| R; GTAAAAAAATAACGCTAATCCGAA |
|
|
| Real-time RT-PCR |
|
|
|
| SOCS1 | F; TGGTAGCACACAACCAGGTG | 92 | [36] |
|
| R; GAGGAGGAAGAGGAGGAAGG |
|
|
| SOCS3 | F; CAAGGACGGAGACTTCGATT | 110 | [36] |
|
| R; GGAGCCAGCGTGGATCTG |
|
|
| GAPDH | F; TGCACCACCAACTGCTTAGC | 87 |
|
|
| R; GGCATGGACTGTGGTCATGAG |
|
|