Characteristics

CEA

MUC1

18s rRNA

NCBI accession number

M29540

NM_002456

X03205

Forward primer

accctggatgtcctctatgg

GTGCCCCCTAGCAGTACCG

gtaacccgttgaaccccatt

Primer length

20

19

20

Amount of use

10 picomol

10 picomol

10 picomol

Reverse primer

caggcataggtcccgttatta

GACGTGCCCCTACAAGTTGG

ccatccaatcggtagtagcg

Primer length

21

20

20

Amount of use

10 picomol

10 picomol

10 picomol

Amplicon length

174

123

152

Optimized annealing temperature

61.4˚C

61.6˚C

53.6˚C