Restriction Enzyme

Recognition Sequence

Aat II

GACGT↓C

Acc II

CG↓CG

Aor13H I

T↓CCGGA

Aor51H I

AGC↓GCT

BspT104 I

TT↓CGAA

BssH II

G↓CGCGC

Cfr10 I

R↓CCGGY

Cla I

AT↓CGAT

Cpo I

CG↓GWCCG

Eco52 I

C↓GGCCG

Hae II

RGCGC↓Y

Hap II

C↓CGG

Hha I

GCG↓C

Mlu I

A↓CGCGT

Nae I

GCC↓GGC

Not I

GC↓GGCCGC

Nru I

TCG↓CGA

Nsb I

TGC↓GCA

PmaC I

CAC↓GTG

Psp1406 I

AA↓CGTT

Pvu I

CGAT↓CG

Sac II

CCGC↓GG

Sal I

G↓TCGAC

Sma I

CCC↓GGG

SnaB I

TAC↓GTA