Reagents

Volume (µl)×1

Final Concentration

Master Mix, 2× conc.

10 µl

1× conc.

Mgcl₂, 25 mM

1.6 µl

2 mM

Primer mix, 20 × conc. {4 µM}

1.0 µl

0.2 µM

Template DNA

5.0 µl

5 - 30 ng

Water, PCR-grade

2.4 µl

-

Total volume

20 µl

-