Gene | Primer sequence | PCR condition | Product size (bp) |
HLA-DRB1*01 | FP:5’TTGTGGCAGCTTAAGTTTGAAT3’ | 94˚C-5 m | 255 |
RP:5’CTGCACTGTGAACGTGTCAC3’ | (94˚C-30 s 51˚C-50 s 72˚C-45 s) × 30 | ||
72˚C-5 m | |||
HLA-DRB1*03 | FR:5’TACTTCCATAACCAGGAGGAGA3’ | 95˚C-2 m | 151 |
RP:5’TGCAGTACTTGTCCACCCG3’ | (95˚C-30 s 53˚C-30 s 72˚C-25 s) × 30 | ||
72˚C-5 m | |||
HLA-DRB1*04 | FR:5’GTTTCTTGGAGCAGGTTAAACA3’ | 95˚C-3 m | 260 |
RP:5’CTGCACTGTGAAGCTCTCAC3 | (95˚C-30 s 53˚C-30 s 72˚C-40 s) × 30 | ||
72˚C-5 m | |||
HLA-DRB1*07 | FR: 5’CCTGTGGCAGGGTAAGTATA3’ | 95˚C-2 m | 232 |
RP:5’CCCGTAGTTGTGTCTGCACAC3’ | (95˚C-30 s 53˚C-30 s 72˚C-25 s) × 30 | ||
72˚C-5 m | |||
HLA-DQB1*0201 | FR:5’GTGCGTCTTGTGAGCAGAAG3’ | 95˚C-2 m | 205 |
RP:5’GCAAGGTCGTGCGGAGCT3’ | (95˚C-30 s 53˚C-30 s 72˚C-25 s) × 30 | ||
72˚C-5 m | |||
HLA-DQB1*0301/4 | FR:5’GACGGAGCGCGTGCGTTA3’ | 95˚C-3 m | 122 |
RP:5’AGTACTCGGCGTCAGGCG3’ | (95˚C-30 s 58˚C-30 s 72˚C-25 s) × 30 | ||
72˚C-5 m |