Gene

Primer sequence

PCR condition

Product size (bp)

HLA-DRB1*01

FP:5’TTGTGGCAGCTTAAGTTTGAAT3’

94˚C-5 m

255

RP:5’CTGCACTGTGAACGTGTCAC3’

(94˚C-30 s 51˚C-50 s 72˚C-45 s) × 30

72˚C-5 m

HLA-DRB1*03

FR:5’TACTTCCATAACCAGGAGGAGA3’

95˚C-2 m

151

RP:5’TGCAGTACTTGTCCACCCG3’

(95˚C-30 s 53˚C-30 s 72˚C-25 s) × 30

72˚C-5 m

HLA-DRB1*04

FR:5’GTTTCTTGGAGCAGGTTAAACA3’

95˚C-3 m

260

RP:5’CTGCACTGTGAAGCTCTCAC3

(95˚C-30 s 53˚C-30 s 72˚C-40 s) × 30

72˚C-5 m

HLA-DRB1*07

FR: 5’CCTGTGGCAGGGTAAGTATA3’

95˚C-2 m

232

RP:5’CCCGTAGTTGTGTCTGCACAC3’

(95˚C-30 s 53˚C-30 s 72˚C-25 s) × 30

72˚C-5 m

HLA-DQB1*0201

FR:5’GTGCGTCTTGTGAGCAGAAG3’

95˚C-2 m

205

RP:5’GCAAGGTCGTGCGGAGCT3’

(95˚C-30 s 53˚C-30 s 72˚C-25 s) × 30

72˚C-5 m

HLA-DQB1*0301/4

FR:5’GACGGAGCGCGTGCGTTA3’

95˚C-3 m

122

RP:5’AGTACTCGGCGTCAGGCG3’

(95˚C-30 s 58˚C-30 s 72˚C-25 s) × 30

72˚C-5 m