Genes/intergenic pacers

Primer Pair (5′-3′)

Amplicon length

T_a (˚C)

Source

atpF-atpH

ACTCGCACACACTCCCTTTCC

GCTTTTATGGAAGCTTTAACAAT

621 bp

48˚C

Ki-Joong Kim; kimkj@KOREA.AC.KR)

rpoc1

GGCAAAGAGGGAAGATTTCG

CCATAAGCATATCTTGAGTTGG

490 bp

53˚C

http://www.kew.org/barcoding/protocols.html

rpoB

ATGCAACGTCAAGCAGTTCC

CCGTATGTGAAAAGAAGTATA

490 bp

51˚C

http://www.kew.org/barcoding/protocols.html

matK

CGTACAGTACTTTTGTGTTTACGAG

CCCAGTCCATCTGGAAATCTTGGTTC

892 bp

49.5˚C

Ki-Joong Kim; kimkj@KOREA.AC.KR)

psbK-psbI

TTAGCCTTTGTTTGGCAAG

AGAGTTTGAGAGTAAGCAT

576 bp

60˚C

Ki-Joong Kim; kimkj@KOREA.AC.KR)

rbcL

GTAAAATCAAGTCCACCRCG ATGTCACCACAAACAGAGACTAAAGC

596 bp

50˚C

David Erickson; ERICKSOND@si.edu)

trnH-psbA

GTTATGCATGAACGTAATGCTC

CGCGCATGGTGGATTCACAATCC

812 bp

50˚C

David Erickson; ERICKSOND@si.edu