Product size

PCR condition

Primer (5´→3´)

Target

759 bp

94˚C, 5 min; 35 cycles of 94˚C, 45 s; 58˚C, 45 s; 72˚C, 60 s

Forward ACGCTGTTGTTAGGAAGTG

Reverse TTGAGGCTGGGTGAAGT

CTX-M

864 bp

94˚C, 2 min; 30 cycles of 95˚C, 45 s; 58˚C, 30 s; 72˚C, 45 s

Forward GGTTAAAAAATCACTGCGTC

Reverse TTGGTGACGATTTTAGCCGC

CTX-M-1 group