Primers and their functions

Sequences (5’-3’)

EcoRI-Adapters 1 and 2

E A 1.1

E A 1.2

MseI-Adapters 1 and 2

M A 1.1

M A 1.2

Preamplification

Pre EcoRI (+0)

Pre MseI (+C)

Selective amplification

EcoRI +C

EcoRI +T

EcoRI +G

MseI +C

CTCGTAGACTGCGTACC

ATTGGTACGCAGTCTAC

GACGATGAGTCCTGAG

TACTCAGGACTCAT

GTAGACTGCGTACCAATTC

ACGATGAGTCCTGAGTAAC

AGACTGCGTACCAATTCC

AGACTGCGTACCAATTCT

AGACTGCGTACCAATTCG

GATGAGTCCTGAGTAAC