| Sl no. | Name of ingredients | Amounts (g/or ml) |
| 1 | Beef extract | 0.5 g |
| 2 | Peptone | 1.0 g |
| 3 | Agar | 1.5 g |
| 4 | NaCl | 1.0 g |
| 5 | Distilled water | 1000 mL |
| Final pH 7.0 with 5N NaOH | ||