Leaf explant

Treatment

Media

CIF (%)

Days for callus induction

Callus morphology

Light

MS + 2,4-D 3.0 mg/l

100

6

Greenish yellow, morphogenic, proliferated

MS + 2,4-D 1.0 mg/l + BAP 3.0 mg/l

80

9

Whitish yellow, morphogenic, proliferated

Dark

MS + 2,4-D 3.0 mg/l

30

16

Brownish yellow, necrotic, non proliferated

MS + 2,4-D 1.0 mg/l + BAP 3.0 mg/l

20

18

Brownish yellow, necrotic, non proliferated

Nodal explant

Light

MS + 2,4-D 3.0 mg/l

30

9

Whitish yellow, morphogenic, proliferated

MS + 2,4-D 1.0 mg/l + BAP 3.0 mg/l

20

15

Brownish yellow, necrotic, non proliferated

Dark

MS + 2,4-D 3.0 mg/l

10

20

Brownish yellow, necrotic, non proliferated

MS + 2,4-D 1.0 mg/l + BAP 3.0 mg/l

10

24

Brownish yellow, necrotic, non proliferated

Inter-nodal explant

Light

MS + 2,4-D 3.0 mg/l

20

10

Whitish yellow, morphogenic, proliferated

MS + 2,4-D 1.0 mg/l + BAP 3.0 mg/l

10

16

Brownish yellow, necrotic, non proliferated

Dark

MS + 2,4-D 3.0 mg/l

10

24

Brownish yellow, necrotic, non proliferated

MS + 2,4-D 1.0 mg/l + BAP 3.0 mg/l

10

25

Brownish yellow, necrotic, non proliferated