Media: MS + 3.0 mg/l 2,4-D

Incubation time

(weeks)

Type of callus

Proliferation response

3

Whitish yellow, soft, friable, granular, less proliferated

++

5

Whitish yellow, soft, friable, granular, less proliferated and morphogenic

+++

8

Whitish yellow, soft, friable, granular, well proliferated, morphogenic

++++

10

Brownish yellow, globular, non-morphogenic, decreased rate of proliferation

+