Media composition (mg/l)

Days for callus induction

Mean No. of cultures showing callus induction

Types of callus

CIF (%)

1.0 mg/l 2,4-D

18

2 ± 0.247

Whitish yellow, granular, soft, less proliferated

20

2.0 mg/l 2,4-D

10

4 ± 0.420

Whitish yellow, granular, friable, morphogenic

40

3.0 mg/l 2,4-D

6

10 ± 0.414

Greenish yellow, granular, morphogenic, soft

100

4.0 mg/l 2,4-D

8

5 ± 0.414

Yellowish brown, globular, morphogenic, rough

50

5.0 mg/l 2,4-D

12

2 ± 0.609

Brown, non-morphogenic, less proliferated

20