Saha S et al, 2020. Complete genome sequence of a novel coronavirus (SARS-CoV-2) isolate from Bangladesh. Microbiol Resour Announc 9: e00568-20.

9 mutations were observed in CHRF_nCoV19_0001, compared to the reference genome for the Wuhan strain (GenBank accession no. MN908947.3) from December 2019. These mutations included the spike protein D614G mutation that is enriched in recent SARS-CoV-2 isolates [15] .


Mahbub et al, 2020. Global and Local Mutations in Bangladeshi SARS-CoV-2 Genomes doi:

Average number of mutations in ORF1ab, S, ORF3a, M and N of genomes, having nucleotide shift at G614 (n = 459), were significantly higher (p ā‰¤ 0.001) than those having mutation at D614 (n = 215). Previously reported frequent mutations such as P4715L, D614G, R203K, G204R and I300F were also prevalent in Bangladeshi isolates. Additionally, 87 unique amino acid changes were revealed [16] .


Study of Transmission, Mutation Rate, Genetic Variants, Non-synonymous Mutation, And Genomic Phylogeny of 263 SARS-CoV-2 Sequenced By Genomic Research Lab, BCSIR. Available at: file:///E:/papers/d%20164%20g/Epidemiological%20Report-BCSIR_Bangladesh_2020%20v1.1.pdf

The highest nucleotide mutations were observed at 112 positions of non-structural protein papain-like protease (nsp3), which led to the 62 non-synonymous amino acid substitutions. Among the structural proteins, the highest mutations were observed at 101 positions of spike proteins resulting in 53 non-synonymous amino acid substitution. The only dominated variant ā€œG614ā€ (due to the change of aspartic acid at 614 number to glycine in spike protein) found in 100% of cases is circulating across the country with co-evolving other variants including L323 (100%) in RNA dependent RNA polymerase (RdRp), K203 and R204 (93.5%) in nucleocapsid, and F120 (83%) in NSP2 [17] .


Mahmud et al, 2020. The genetic variants analysis of circulating SARS-CoV-2 in Bangladesh. DOI: 10.1101/2020.07.29.226555

Highest mutations at 36 different positions have identified in spike proteins. A total of 9 mutations at spike proteins were found unique in relative to the global mutations including mutations at position 516 in the boundary of the ACE2 binding region of the spike protein [18] .