Culture conditions

LB

LB + 3% Ethanol

Starter OD_600nm^(*)

2.8^(o/n)

0.5

2.8^(o/n)

0.5

1.9^(o/n)

1.9^(o/n)

0.5

Expansion OD_600nm^(**)

0.4

0.8

0.4

0.8

0.4

1.0

0.4

Temp (˚C)

37

37

26

26

37

26

26

Time (h)

4

4

24

24

4

24

24