Component

Length (base pairs)

5’ to 3’ Sequence

Reaction Mix DNA

450

CTTACGACCAGTCGTATGCCTTCTCGAGTTCCGTCCGGTTAAGCGTGACAGTCCCAGTGAACCCACAAACCGTGATGGCTGTCCTTGGAGTCATACGCAAGAAGGATGGTCTCCAGACACCGGCGCACCAGTTTTCACGCCGAAAGCATAAACGACGAGCACATATGAGAGTGTTAGAACTGGACGTGCGGTTTCTCTGCGAAGAACACCTCGAGCTGTTGCGTTGTTGCGCTGCCTAGATGCAGTGTCGCACATATCACTTTTGCTTCAACGACTGCCGCTTTCGCTGTATCCCTAGACAGTCAACAGTAAGCGCTTTTTGTAGGCAGGGGCTCCCCCTGTGACTAACTGCGCCAAAACATCTTCGGATCCCCTTGTCCAATCTAACTCACCGAATTCTTACATTTTAGACCCTAATATCACATCATTAGAGATTAATTGCCACTGCCA

Reaction Mix Forward Primer

20

TCTCGAGTTCCGTCCGGTTA

Reaction Mix Reverse Primer

20

CTCCAAGGACAGCCATCACG

Melting DNA

69

GCCGTAAACGGTTATATAGCCCGATTAAATTATATCGCCCGGCCCTTAAAATAAGTAATTCGCGGCG