| Component | Concentration |
| Tris | 50 mM |
| MgCl2 | 3 mM |
| Each dNTP (dATP, dCTP, dGTP, dTTP) | 200 µM |
| Nonacetylated BSA (Sigma) | 500 µg/mL |
| LCGreen® Plus (BioFire Diagnostics) | 1´ |
| Melting DNA (see Table 4) | 2 µM |