Component

Concentration

Tris

50 mM, pH 8.3

MgCl₂

5 mM

Each dNTP

(dATP, dCTP, dGTP, dTTP)

200 µM

Nonacetylated BSA

(Sigma)

500 µg/mL

LCGreen® Plus

(BioFire Diagnostics)

1´

Klentaq1™ DNA polymerase

2 µM

Reaction Mix DNA

(see Table 4)

2100 copies/µL

Reaction Mix Forward Primer

(see Table 4)

5 µM

Reaction Mix Reverse Primer

(see Table 4)

5 µM