Time (min)

concentrations

12.5 mM

25 mM

50 mM

25

−2.31 ± 2.28

22.62 ± 8.70**

91.36 ± 9.26+++

50

−2.66 ± 1.75

25.01 ± 1.92***

235.27 ± 15.62+++

75

−1.02 ± 1.39

69.14 ± 4.94***

316.14 ± 15.99+++

100

0.04 ± 1.00

102.96 ± 7.27***

344.47 ± 18.19+++

125

3.39 ± 2.33

123.24 ± 9.00***

363.16 ± 21.55+++

150

2.35 ± 3.46

142.39 ± 8.86***

380.07 ± 22.28+++

175

6.68 ± 1.42

177.12 ± 13.25***

392.54 ± 26.29+++

200

6.76 ± 2.91

208.34 ± 15.21***

421.39 ± 32.15+++

225

11.09 ± 3.28

205.15 ± 17.84***

433.89 ± 33.50+++

250

11.90 ± 1.81

230.52 ± 21.55***

452.65 ± 41.50+++

275

8.12 ± 1.66

250.21 ± 20.14***

478.95 ± 48.79++

300

7.70 ± 2.84

277.94 ± 21.77***

496.54 ± 54.71++