Time (min) | concentrations | ||
12.5 mM | 25 mM | 50 mM | |
25 | −2.31 ± 2.28 | 22.62 ± 8.70** | 91.36 ± 9.26+++ |
50 | −2.66 ± 1.75 | 25.01 ± 1.92*** | 235.27 ± 15.62+++ |
75 | −1.02 ± 1.39 | 69.14 ± 4.94*** | 316.14 ± 15.99+++ |
100 | 0.04 ± 1.00 | 102.96 ± 7.27*** | 344.47 ± 18.19+++ |
125 | 3.39 ± 2.33 | 123.24 ± 9.00*** | 363.16 ± 21.55+++ |
150 | 2.35 ± 3.46 | 142.39 ± 8.86*** | 380.07 ± 22.28+++ |
175 | 6.68 ± 1.42 | 177.12 ± 13.25*** | 392.54 ± 26.29+++ |
200 | 6.76 ± 2.91 | 208.34 ± 15.21*** | 421.39 ± 32.15+++ |
225 | 11.09 ± 3.28 | 205.15 ± 17.84*** | 433.89 ± 33.50+++ |
250 | 11.90 ± 1.81 | 230.52 ± 21.55*** | 452.65 ± 41.50+++ |
275 | 8.12 ± 1.66 | 250.21 ± 20.14*** | 478.95 ± 48.79++ |
300 | 7.70 ± 2.84 | 277.94 ± 21.77*** | 496.54 ± 54.71++ |