Primers

Initial denaturation

30 Cycles

Final denaturation

Denaturation

Hybridization

Elongation

Temperature and time of operations

CkFKSf/CkFKSr

95˚C 5 min−1

94˚C 45 s−1

50˚C 45 s−1

72˚C 1min−1

72˚C 10 min−1

SC-5fw/SC-3bw

95˚C 5 min−1

94˚C 45 s−1

50˚C 45 s−1

72˚C 1min−1

72˚C 10 min−1

LbF/LbR

95˚C 5 min−1

94˚C 45 s−1

60˚C 45 s−1

72˚C 1min−1

72˚C 10 min−1

LcF/LcR

95˚C 5 min−1

94˚C 45 s−1

55˚C 45 s−1

72˚C 1min−1

72˚C 10 min−1

B-K1/5F, B-K1/3R

95˚C 5 min−1

95˚C 30 s−1

55˚C 30 s−1

72˚C 1min−1

72˚C 7 min−1