Reagents

Initial

concentration

Final

concentration

Quantity (µl)

for 1 reaction

Quantity (µl) for 8 reactions

(master mix for each primer set)

Red mix

2×

1×

12.5 µl

100 µl

Forward primer

10 µM

0.2 µM

0.5 µl

4 µl

Reverse primer

10 µM

0.2 µM

0.5 µl

4 µl

Template DNA

-

-

1 µl

-

Distilled water

-

-

10.5 µl

84 µl

Total

-

-

25 µl

192 µl