| Template DNA | Primer set 1 | Primer set 2 |
| Samples | Tubes from 1 to 4 | Tubes from 1 to 4 |
| Positive control | Tube 5 | Tube 5 |
| Negative control | Tube 6 | Tube 6 |
| No-template control | Tube 7 | Tube 7 |