Inductor

Origin of CMMs

Steps

Time

Percentage of differentiation reported

Technique used to demonstrate differentiation

Functionality demonstration

Reference

5azadC and TSA

Balb/c mouse bone marrow

Pre-induction with 25 ng/ml of bFGF and EGF for 2 days.

Subsequent induction with 1 mM of 5azadC and 40 nM of TSA.

2 days

70% demonstrated by morphological changes of neuronal nature

Neuronal morphology was observed under a microscope.

Increased expression of Sox2 by RT-PCR.

Immunostaining for Tuj1 and GFAP.

No

[67]