| Co-culture with human Schwann cells | Adipose tissue of adult humans | CMM was co-cultured with human Schwann cells from benign Schwannomas of the acoustic nerve. | 7 days | 25% demonstrated by positive immunofluorescence for PMP-22. | Glial morphology was observed under a microscope. Immunostaining for PMP-22 and S-100.
| No | [94]
|
| Culture in conditioned medium with neurons of mouse cerebellar granules. | The bone marrow of adult Wistar rat. | CMM was cultured in a conditioned medium with C57BL/6 mouse cerebellar granules neurons. | 5 days | 40% and 19% were demonstrated by positive immunofluorescence for GFAP and NeuN, respectively. | Neuronal morphology was observed under a microscope. Immunostaining for Tuj1, NeuN, and GFAP.
| Negative resting membrane potential and presence of voltage-gated Na+ and K+ channels. Ability to evoke electric currents in response to GABA, glycine, and glutamate, demonstrated by Patch-clamp. | [95] |
| Medium conditioning of olfactory enveloping cells of Wistar rats. | Adipose tissue of adult humans | The CMMs were co-cultured with olfactory envelope cells isolated from the olfactory bulb of a 2-day-old Wistar rat. | 7 days | 95%, 46%, 30% and 72% demonstrated by positive immunofluorescence for PGP9.5, MAP2, Synapsin I, and GFAP, respectively. | Neuronal morphology was observed under a microscope. Immunostaining for Nestin, PGP 9.5, MAP2, Synapsin I, and GFAP. | No | [96] |