Screening approach

Target gene

Detection method

Inducer

Source

Host, vector

Agar plate screening

b-Glycosidases

Phenotypical detection

AZCL-xylan, xyloglucan

Cow dung

E. coli, phage

Agar plate screening

Genes resistant to toxic elements

Phenotypical detection

Several antibiotics

Dairy cow manure

E. coli, fosmid

Agar plate screening

Genes resistant to toxic elements

Phenotypical detection

Several antibiotics

Cheese food matrix

E. coli, fosmid

Microtiter plate screening

Cellulase

Absorbance measurement

Dinitrophenol-cellobioside

Soil, Buffalo rumen, etc.

E. coli, fosmid

GMD, FACS

Screening for antibiotics

Fluorescence

S. aureus

3 strains of Staphylococcus obtained from an ARSculture collection

E. coli S. cerevisiae, plasmid

Microfluidics (water in oil droplets), FACS

Hydrolases

Fluorescence

Sulfate monoester

Phosphate triester

Variety of sources (soil, degraded plant material, cow rumen)

E. coli, plasmid