Ref. | Evaluation method | Host animal | Output |
[24] | Comparison of the bacterial profile of intracellular (iDNA) and extracellular DNA (eDNA) Rumen fluid treatment (cheesecloth squeezed, centrifuged filtered), Storage temperature (RT, −80˚C) and Cryo protectants (PBS-glycerol, ethanol) | cow rumen | Intracellular DNA extraction using bead-beating method from cheesecloth sieved rumen content mixed with PBS-glycerol and stored at −80˚C was found as the optimal method to study ruminal bacterial profile. |
[22] | Fifteen different DNA extraction methods | cow and sheep rumen | There is significant differences in microbial community between extraction methods, e.g. Relative abundances some bacteria e.g. phyla Bacteroidetes and Firmicutes DNA extraction methods that involved phenol-chloroform extraction and mechanical lysis steps tended to be more comparable. |
[25] | DNA extraction such as: Repeated bead beating (RBB), Phenol dependent bead beating (PBB), Fast spin DNA kit for soil (FDSS), and PQIAmini. On observed microbial communities from fibrous and liquid rumen fractions | Dairy cows. | All four extraction procedures yielded DNA suitable for further analysis of bacterial, archaeal and anaerobic fungal communities using quantitative PCR and pyrosequencing of relevant taxonomic markers. |
[26] | Ten improved DNA extraction methods | Yak | hexadecyltrimethylammomium bromide-lysozyme using physical lysis by bead beating is recommended for the DNA isolation of the rumen microbial community. It also showed that the bead-beating step is necessary to effectively break down the cell walls of all of the microbes, especially Gram-positive bacteria. |