Ref. | Evaluation method | Host animal | Out put |
[21] | Sampling technique (cannulation vs. stomach tube) and Site (dorsal sac vs. ventral sac) on the rumen microbiome and fermentation parameters | Han woo steers. | Rumen microbiome and fermentation parameters are not affected by different sampling techniques and sampling sites. A stomach tube can be a feasible alternative method to collect representative rumen samples. |
[20] | Processing method rumen liquor that was either immediately frozen or samples that were stored as cell pellets on the key microbial group | Fistulated Brahman steers | Regardless of the processing method used, both identified the key microbial groups. However, immediately freezing samples might alter the abundance of species |