Ref.

Evaluation method

Host animal

Out put

[21]

Sampling technique

(cannulation vs. stomach tube) and

Site

(dorsal sac vs. ventral sac)

on the rumen microbiome and fermentation parameters

Han woo steers.

Rumen microbiome and fermentation parameters are not affected by different sampling techniques and sampling sites.

A stomach tube can be a feasible alternative method to collect representative rumen samples.

[20]

Processing method

rumen liquor that was either immediately frozen or samples that were stored as cell pellets on the key microbial group

Fistulated Brahman steers

Regardless of the processing method used, both identified the key microbial groups.

However, immediately freezing samples might alter the abundance of species