| Transcripts | Primer Sequences | PCR Programs | Cycles |
| p53 | F-5’CGGAGGTCGTGAGACGCTG’3 R-5’CACATGTACTTGTAGTGGATGGTGG’3 | 94˚C-1m/59˚C-1m/72˚C-1m | 40 |
| Foxo1 | F-5’GTGAACACCATGCCTCACAC’3 R-5’CACAGTCCAAGCGCTCAATA’3 | 95˚C-15s/60˚C-1m/72˚C-1m | 40 |
| Ins-1 | F-5’CCTGCTCGTCCTCTGGGAGCCCAAG’3 R-5’CTCCAGTGCCAAGGTCTGAAGATCC’3 | 94˚C-1m/68˚C-1m/72˚C-1m | 35 |
| Ins-2 | F-5’CCTGCTCATCCTCTGGGAGCCCCGC’3 R-5’CTCCAGTGCCAAGGTCTGAAGGTCA’3 | 94˚C-1m/70˚C-1m/72˚C-1m | 40 |
| Sirt-1 | F-5’AGGGAACCTCTGCCTCATCTAC’3 R-5’GGCATACTCGCCACCTAACCT’3 | 94˚C-1m/60˚C-1m/72˚C-1m | 35 |
| Bcl-2 | F-5’CAGCTGCACCTGACGCCCTT’3 R-5’CCCAGCCTCCGTTATTCTGGA’3 | 94˚C-1dk/58˚-1dk/72˚C-1dk | 40 |
| GAPDH | F-5’GTCGTGGAGTCTACTGGCGTC’3 R-5’GATGACCCTTTTGGCACCACC’3 | 94˚C-1m/54˚C-1m/72˚C-1m | 35 |