| Method | Sitting-drop vapor diffusion |
| Plate type | 24-well |
| Temperature (K) | 295 |
| Protein concentration (mg・mL−1) | 15 |
| Buffer composition of protein solution | 10 mM Tris-HCl pH8.0, 200 mM NaCl |
| Composition of reservoir solution | 7% PEG 4000, 0.2 M Sodium Acetate 0.1 M MES pH6.5 |
| Volume and ratio of drop | 1:1 |
| Volume of reservoir (mL) | 0.5 |