Est1-dm | Est1-dm | Est1-dm | |
Culture conditions | 25˚C O/N | 37˚C・6h | 37˚C O/N |
Culture volume (mL) | 10 | 10 | 10 |
IPTG concentration (mM) | 0.1 | 0.1 | 0.1 |
Harvested cells (g) | 0.3 | 0.2 | 0.4 |
Eluted volume (mL) | 5 | 5 | 5 |
Protein concentration (mg/mL) | 2 | 1 | 0.12 |
Total protein (mg) | 10 | 5 | 0.6 |