Source organism

Thermobifida alba

DNA source

Genbank AB445476

Forward primer

5’-CGCGGATCCAACCCCTACGAACGCGGC-3’

Reverse primer

5’-GCGAAGCTTGAACGGGCAGGTGGAGCGGT-3’

Cloning vector

pGEM-T

Expression vector

pQE80L-est1

Expression host

E. coli Rosetta-gami (DE3)

Complete amino acid sequence of the construct produced

MRGSHHHHHHGSNPYERGPNPTESMLEARSGPFSVS

EERVSRLGADGFGGGTIYYPRENNTYGAIAISPGYTG

TQSSIAWLGERIASHGFVVIAIDTNTTLDQPDSRARQL

NAALDYMLTDASSSVRNRIDASRLAVMGHSMGGGG

TLRLASQRPDLKAAIPLTPWHLNKSWRDITVPTLIIGA

DLDTIAPVSSHSEPFYNSIPSSTDKAYLELNNATHFAPN

IPNKTIGMYSVAWLKRFVDEDTRYTQFLCPGPRTGLLS

DVDEYRSTCPF