| Primer | Sequence (5'-3') | Purpose |
| Oligo dT | CTGTGAATGCGACTACGATTTTTTTTTTTTTTTTTTT | cDNA synthesis |
| Chi-a* | GGNGGNTGGAAYATGGG | Primary PCR |
| Chi-b* | TNGCNGCNTTYGARTGGAAYGA | Primary PCR |
| Chi-c* | ANCANRANCCNTTRGTYACCCA | Primary PCR |
| SeChi-1-1 | GCACCAAAGAAAAAAGTTGAT | 3'RACE |
| SeChi-2-1 | GGACATAACAGCCCTCTG | 3'RACE |
| 3R | CTGTGAATGCGACTACGAT | 3'RACE |
| SeChi-1-2 | GCCCATGTTCCAGCCACC | 5'RACE |
| SeChi-1-3 | TTCGTCGTTCCACTCAAAAGC | 5'RACE |
| SeChi-1-4 | CCCATTCAGTTTGGCAAAAGC | 5'RACE |
| SeChi-2-2 | CGCTACCATGGCAGTGAAAGG | 5'RACE |
| SeChi-2-3 | CTTCATCCATGGTTCAGATTC | 5'RACE |
| AAP | GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG | 5'RACE |
| AUAP | GGCCACGCGTCGACTAGTAC | 5'RACE |
| SeChi-1-5 | ACACATTACAGCAAA | Full-length ORF |
| SeChi-1-6 | TAATAAATACCAAGATTAT | Full-length ORF |
| SeChi-2-4 | CGAGTTCTGGTGGACAAA | Full-length ORF |
| SeChi-2-5 | GGCTGAAAAATAAAATGT | Full-length ORF |
| β-actin-a | GGTATGTGCAAAGCTGGTTTT | Organ expression |
| β-actin-b | GTGGGTGACACCATCACCAGA | Organ expression |
| SeChi-1-a | GAAACTTTGATGGTTTGGACAT | Organ expression |
| SeChi-1-b | TGTGTCCATACAAAGCAATTCC | Organ expression |
| SeChi-2-a | GAGAAATACCCACTGCTGAAGA | Organ expression |
| SeChi-2-b | AGAACAGTTAGGAATAGCGGAT | Organ expression |