Steps (Flow rate)

AFFINIMIP SPE BPA (100 mg/6 ml)

1) Equilibration with 2 drops/s

3 ml Methanol (2% Acetic acid)

3 ml Acetonitrile

3 ml Water

2) Loading (ml) 1 drop/2 s

20 ml of diluted filtrate

3) Washing of interferents (1 drop/s)

9 ml of ultrapure water

6 ml of Water-Acetonitrile (60:40, v/v)

4) Drying

Force the water down and out the bottom, apply vacuum for 30 s

5) Elution (1 drop/s)

3 ml of Methanol