Steps (Flow rate) | AFFINIMIP SPE BPA (100 mg/6 ml) |
1) Equilibration with 2 drops/s | 3 ml Methanol (2% Acetic acid) 3 ml Acetonitrile 3 ml Water |
2) Loading (ml) 1 drop/2 s | 20 ml of diluted filtrate |
3) Washing of interferents (1 drop/s) | 9 ml of ultrapure water 6 ml of Water-Acetonitrile (60:40, v/v) |
4) Drying | Force the water down and out the bottom, apply vacuum for 30 s |
5) Elution (1 drop/s) | 3 ml of Methanol |