| Plasmid Inserts | Length (base pairs) | GC Content % | Nearest Neighbor ˚C | Tm basic ˚C | Comment |
| 1 | 143 | 57.0 | 84.92 | 83.7 | A |
| 2 | 313 | 35.5 | 79.86 | 77.3 | A |
| 3 | 153 | 44.0 | 79.98 | 78.5 | A |
| 4 | 153 | 51.0 | 82.27 | 81.4 | A |
| 5 | 191 | 54.0 | 83.76 | 83.5 | A |
| 6 | 243 | 50.0 | 84.49 | 82.5 | A |
| 7 | 243 | 50.0 | 84.49 | 82.5 | A |
| 8 | 243 | 50.0 | 84.49 | 82.5 | A |
| 9 | 261 | 54.0 | 85.39 | 84.5 | A |
| 10 | 241 | 49.8 | 84.45 | 82.5 | A |
| 11 | 241 | 49.8 | 84.45 | 84.1 | A |
| 12 | 334 | 52.0 | 84.98 | 84.1 | A |
| 13 | 319 | 59.0 | 87.47 | 86.8 | A |
| 14 | 146 | 59.0 | 86.18 | 84.4 | A |
| 15 | 308 | 35.0 | 79.70 | 77.0 | A |
| 16 | 312 | 35.3 | 79.88 | 77.2 | A |
| 17 | 101 | 54 | 83.39 | 80.6 | A |
| 18 | 69 | 55 | 78.24 | 77.7 | A |
| 19 | 341 | 37 | 80.79 | 78.3 | A |
| 20 | 121 | 55 | 82.99 | 82.0 | A |
| 21 | 159 | 55 | 84.27 | 83.3 | B |
| 22 | 302 | 50 | 85.17 | 83.3 | B |
| 23 | 199 | 59.0 | 87.74 | 85.8 | B |
| 24 | 365 | 50.7 | 86.28 | 84.0 | B |
| 25 | 365 | 51.2 | 86.37 | 84.0 | B |
| 26 | 188 | 59.0 | 87.74 | 85.8 | B |
| 27 | 292 | 51.0 | 84.67 | 83.5 | B |
| 28 | 166 | 59.0 | 86.18 | 84.4 | B |
| 29 | 442 | 60.0 | 89.4 | 87.8 | B |