| Runs | Substrate (g) | Moisture (%) | Temperature (°C) | PA* (U/mL) |
| 1 | 3 | 30 | 25 | 353.83 |
| 2 | 7 | 30 | 25 | 337.00 |
| 3 | 3 | 50 | 25 | 353.67 |
| 4 | 7 | 50 | 25 | 383.17 |
| 5 | 3 | 30 | 35 | 312.50 |
| 6 | 7 | 30 | 35 | 235.17 |
| 7 | 3 | 50 | 35 | 251.17 |
| 8 | 7 | 50 | 35 | 263.67 |
| 9 (C) | 5 | 40 | 30 | 399.17 |
| 10(C) | 5 | 40 | 30 | 404.67 |
| 11(C) | 5 | 40 | 30 | 397.67 |
| 12(C) | 5 | 40 | 30 | 404.17 |