| Total activity (Utot) | Total protein (mg) | Specific activity (U) | Purification fold | Yield (%) |
Crude extract | 125.3 | 3.16 | 39.6 | 1.0 | 100.0 |
(NH4)2SO4 | 102.8 | 0.81 | 126.9 | 3.2 | 82.0 |
Gel filtration | 76.6 | 0.15 | 510.9 | 12.9 | 61.2 |
Cation exchange | 61.1 | 0.05 | 1221.4 | 30.8 | 48.8 |